使用Architect HIV Ag / Ab Combo的样本与截止值的比率：补充测试结果和预测HIV感染的最佳截止值的影响。,Journal of Clinical Laboratory Analysis

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使用Architect HIV Ag / Ab Combo的样本与截止值的比率：补充测试结果和预测HIV感染的最佳截止值的影响。
Journal of Clinical Laboratory Analysis ( IF 2.6 ) Pub Date : 2019-02-25 , DOI: 10.1002/jcla.22866
Linchuan Wang ₁ , Jing-Yuan Wang ₁ , Xu-Dong Tian ₁ , Jin-Xiong Ruan ₁ , Yan Yu ₂ , Fang Yan ₃

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背景技术建筑师HIV Ag / Ab Combo具有出色的HIV筛查性能。但是，在艾滋病毒感染率较低的情况下，假阳性率（FPR）较高。目的本研究的目的是分析建筑师HIV Ag / Ab Combo的样品与截止（s / co）比率的影响以及确认测试的结果，并探索s / co预测HIV感染的潜在效用。方法在西安市的一家教学医院对建筑师HIV Ag / Ab组合反应结果进行回顾性审查。比较了不同组的s / co值，即WB阳性病例之间的真阳性（TP）和假阳性（FP），不同的Western blotting（WB）谱带。接受者工作特征曲线（ROC）分析用于确定预测HIV感染的最佳临界值。结果在研究期间，ARCHITECT对84 702名患者中的219名有反应，HIV患病率为0.0992％，FPR为56.25％。TP的平均s / co比明显高于FP（458.15 vs 3.11，P <0.0001）。在WB阳性病例中，s / co比随带数的增加而显着增加，P = 0.0065。ROC分析得出的最佳临界值（24.44）可以提供最高的灵敏度（100％）和特异性（100％）之和，而无FP结果。结论对于建筑师HIV Ag / Ab组合，当s / co比增加时，FPR会降低，并且s / co≥24.44可以可靠地预测HIV感染。P <0.0001）。在WB阳性病例中，s / co比随带数的增加而显着增加，P = 0.0065。ROC分析得出的最佳截止值（24.44）可以提供最高的灵敏度（100％）和特异性（100％）之和，而无FP结果。结论对于建筑师HIV Ag / Ab组合，当s / co比增加时，FPR会降低，并且s / co≥24.44可以可靠地预测HIV感染。P <0.0001）。在WB阳性病例中，s / co比随带数的增加而显着增加，P = 0.0065。ROC分析得出的最佳截止值（24.44）可以提供最高的灵敏度（100％）和特异性（100％）之和，而无FP结果。结论对于建筑师HIV Ag / Ab组合，当s / co比增加时，FPR会降低，并且s / co≥24.44可以可靠地预测HIV感染。

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Sample-to-cutoff ratios using Architect HIV Ag/Ab Combo: The influence with the results of supplemental tests and optimal cutoff value to predict HIV infection.

BACKGROUND The Architect HIV Ag/Ab Combo has excellent performance for HIV screening; however, the false-positive rate (FPR) was high in low HIV prevalence setting. OBJECTIVES The purpose of this study was to analyze the influence of sample-to-cutoff (s/co) ratios by Architect HIV Ag/Ab Combo with the results of confirmatory test and explore the potential utility of s/co to predict HIV infection. METHODS A retrospective review on Architect HIV Ag/Ab Combo reactive results was performed at a teaching hospital in Xi'an. The s/co values in different groups, that is, true positives (TP) and false positives (FP), different Western blotting (WB) bands among WB-positive cases, were compared. The receiver operating characteristic curve (ROC) analysis was used to determine the optimal cutoff value for predicting HIV infection. RESULTS During the study period, 219 out of 84 702 patients were reactive by ARCHITECT with a 0.0992% of HIV prevalence and a 56.25% of FPR. The mean s/co ratios in TP were significantly higher than that in FP (458.15 vs 3.11, P < 0.0001). Among the WB-positive cases, the s/co ratios increased significantly with the increase in the number of bands, P = 0.0065. The optimal cutoff (24.44) by ROC analysis can provide the highest sum of sensitivity (100%) and specificity (100%) with no FP results. CONCLUSIONS For Architect HIV Ag/Ab Combo, the FPR is reduced when s/co ratios increase, and the s/co ≥24.44 may be reliable to predict HIV infection.

更新日期：2019-11-01

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