A near-infrared fluorescence probe for hydrazine based on dicyanoisophorone                        
Microchemical Journal  Pub Date : 2020-05-30 , DOI:  10.1016/j.microc.2020.105066 
Shiqing Zhang, Daiwen Chen, Liqiang Yan, Ya Xie, Xinyue Mu, Jinbiao Zhu

With the widespread use of N₂H₄, it brings potential risks to human health. In order to determine N₂H₄ effectively in both environment and living systems, a simple near-infrared fluorescence probe was prepared for the specific response of N₂H₄ based on the intramolecular charge transfer (ICT) mechanism. This probe provided a “turn on” fluorescence response to N₂H₄ with characteristics including high selectivity, rapid response (within 1 min), low cytotoxicity, and low detection limit (0.022 μM). In addition, it can be used to expediently trace N₂H₄ in HepG2 cells and water samples. Thus, Probe 1 is a promising tool to identify the level of N₂H₄.