研究兴趣：酶的结构解析与酶催化机制的计算分析

文章发表：

1. Zhiwei Deng, Zhenbo Yuan, Zhengshan Luo, Yijian Rao*, BTG13-related metalloenzymes: atypical non-heme iron-dependent dioxygenases with unusual coordination patterns and catalytic mechanisms, Engineering Microbiology, 2025, 100188.

2. Z.W. Deng#, H. Su#, X.D. Hou, H.B. Xu, Z.B. Yuan, X. Sheng*, Y.J. Rao*,  Mechanistic Investigation on C–C Bond Cleavage of Anthraquinone Catalyzed by an Atypical Non-heme Iron-dependent Dioxygenase BTG13, ACS Catal, 2024, 14(2), 797–811.

3. Lifeng Yang, Mengliang Yang, Zhiwei Deng, Zhengshan Luo, Zhenbo Yuan, Yijian Rao, Yan Zhang, Highly Efficient Biosynthesis of Rebaudioside M8 through Structure-Guided Engineering of Glycosyltransferase UGT94E13, J Agri Food Chem, 2024, 72 (28), 15823-15831

4.  Y. Gao, F. Li, Z.S. Luo, Z.W. Deng, Y. Zhang, Z.B. Yuan, C.M Liu, and Y.J. Rao*, Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids, Nature Commun, 2024, 15:30.

5.  H.B. Xu#, Z.B. Yuan#, S. Yang#, Z.P. Su, X.D. Hou, Z.W. Deng, Y. Zhang, Y.J. Rao*, Discovery of a fungal P450 with an unusual two-step mechanism for constructing bicyclo[3.2.2]nonane skeleton, JACS, 2024, 146 (12), 8716-8726.

6. Z. P. Su, B.D. Guo, H.B. Xu, Z.B. Yuan, H.L. Liu, T. Guo, Z.W. Deng, Y. Zhang, D.J. Yin, C.M. Liu, J.H. Chen,  Y.J. Rao*, Synthetic Biology-based Construction of Unnatural Perylenequinones with Improved Photodynamic Anticancer Activities, Angew, 2024, e202317726. 

7. L.F. Yang, M.L. Yang, Z.W. Deng, X.D. Hou, X.T.  Zheng, Q. Ping, Y.J. Rao, J.S. Shi, Y. Zhang, Selective synthesis of rebaudioside M2 through structure-guided engineering of glycosyltransferase UGT94D1, Front. Bioeng. Biotechnol, 2024, 5:12:1334427.

8. D. Li^#, Z.W. Deng^#, X.D. Hou, Z.J. Qin, X.L. Wang, D.J. Yin, Y. Chen, Y.J. Rao, J. Chen, J.W. Zhou*, Structural Insight into the Catalytic Mechanisms of an L‐Sorbosone Dehydrogenase, Advanced Science, 2023,10(30):e2301955

9. C.T. Zhu, Z.B. Yuan*, Z.W. Deng, D.J. Yin, Y. Zhang, J.W. Zhou*, Y.J. Rao*, Photoenzymatic Enantioselective Synthesis of Oxygen-Containing Benzo-Fused Heterocycles, Angew, 2023, e202311762.

10. X.Z. Liu, Z.B. Yuan, H. Su, X.D. Hou, Z.W. Deng, H.B. Xu, B.D. Guo, D.J. Yin, X. Sheng, Y.J. Rao*, Molecular Basis of the Unusual Seven-membered Methylenedioxy Bridge Formation Catalyzed by Fe(II)/α-KG-dependent Oxygenase CTB9, ACS Catal, 2022, 12 (6), 3689-3699.

11. X.D. Hou#, H.B. Xu#, Z.W. Deng, Y.J. Yan, Z.B. Yuan, X.Z. Liu, Z.P. Su, S. Yang, Y. Zhang, Y.J. Rao*, Discovery of the Biosynthetic Pathway of Beticolin 1 Reveals a Novel Non-heme Iron-dependent Oxygenase for Anthraquinone Ring Cleavage, Angew, 2022, online.

12. X.D. Hou#, H.B. Xu# Z.B. Yuan, Z.W. Deng, K. Fu, Y. Gao, C.M. Liu, Y. Zhang, Y.J. Rao*, Structural analysis of an anthrol reductase inspires enantioselective synthesis of enantiopure hydroxycycloketones and β-halohydrins, Nature Communications, Accepted, 2023.

13. S. Yang, X.D. Hou, Z.W. Deng, L.F. Yang, Q. Ping, Z.B. Yuan, Y. Zhang, Y.J. Rao*, Improving the Thermostability of Glycosyltransferase YojK by Targeting Mutagenesis for Highly Efficient Biosynthesis of Rebaudioside D, Molecular Catalysis, 2023, online.

14. B.D. Guo, X.D. Hou, Y. Zhang, Z.W. Deng, Q. Ping, K. Fu, Z.B. Yuan, Y.J. Rao*, Highly Efficient Production of Rebaudioside D Enabled by Structure-guided Engineering of Bacterial Glycosyltransferase YojK,Front Bioeng Biotechnol, 2022, accepted.

15. B.D. Guo, Z.W. Deng, F. Meng, Q.F. Wang, Y. Zhang, Z.B. Yuan, Y.J. Rao*, Enhancement of Rebaudioside M Production by Structure-guided Engineering of Glycosyltransferase UGT76G1, J Agric Food Chem，Accepted, 2022

16. H. Huang, X.D. Hou, R.R. Xu, Z.W. Deng, Y. Wang, G.C. Du, Y.J. Rao*, J. Chen, Z. Kang, Structure and cleavage pattern of a hyaluronate 3-glycanohydrolase in the glycoside hydrolase 79 family, Carbohyd Polym, 277 (2022).