研究兴趣：新酶催化反应的挖掘与机制解析

文章发表：

1. X.D. Hou#, H.B. Xu#, Z.W. Deng, Y.J. Yan, Z.B. Yuan, X.Z. Liu, Z.P. Su, S. Yang, Y. Zhang, Y.J. Rao*, Discovery of the Biosynthetic Pathway of Beticolin 1 Reveals a Novel Non-heme Iron-dependent Oxygenase for Anthraquinone Ring Cleavage, Angew, 2022, online.

2. X.D. Hou#, H.B. Xu# Z.B. Yuan, Z.W. Deng, K. Fu, Y. Gao, C.M. Liu, Y. Zhang, Y.J. Rao*, Structural analysis of an anthrol reductase inspires enantioselective synthesis of enantiopure hydroxycycloketones and β-halohydrins, Nature Communications, Accepted, 2023.

3. X.Z. Liu^#, Z.B. Yuan^#, H. Su^#, X.D. Hou^#, Z.W. Deng, H.B. Xu, B.D. Guo, D.J. Yin, X. Sheng, Y.J. Rao*, Molecular Basis of the Unusual Seven-membered Methylenedioxy Bridge Formation Catalyzed by Fe(II)/α-KG-dependent Oxygenase CTB9, ACS Catal, 2022, 12 (6), 3689-3699

4. X. Xu#, S.H. Yan#, X.D. Hou#, W. Song, L. Wang, T.F. Wu, M.Y. Qi, J. Wu, Y.J. Rao*, B.J. Wang, L.M. Liu, Local Electric Field Modulated Reactivity of Pseudomonas aeruginosa Acid Phosphatase for Enhancing Phosphorylation of L-Ascorbic Acid, ACS Catal, 11 (2021) 13397-13407.

5. B.D. Guo#, X.D. Hou#, Y. Zhang, Z.W. Deng, Q. Ping, K. Fu, Z.B. Yuan, Y.J. Rao*, Highly Efficient Production of Rebaudioside D Enabled by Structure-guided Engineering of Bacterial Glycosyltransferase YojK, Front Bioeng Biotechnol, 2022, accepted.

6. S. Yang, X.D. Hou, Z.W. Deng, L.F. Yang, Q. Ping, Z.B. Yuan, Y. Zhang, Y.J. Rao*, Improving the Thermostability of Glycosyltransferase YojK by Targeting Mutagenesis for Highly Efficient Biosynthesis of Rebaudioside D, Molecular Catalysis, Accepted.

7. D Ma, Z Cheng, L Peplowski, L Han, Y Xia, X Hou, J Guo, D Yin, Y Rao*, Z Zhou*, Insight into the broadened substrate scope of nitrile hydratase by static and dynamic structure analysis, Chemical Science, 2022, online
   

8. Y.Q. Tian, X.D. Hou, D.W. Ni, W. Xu, C. Guang, W.L. Zhang, Q.M. Chen, Y.J. Rao*, W.M. Mu*, Structure-based interface engineering methodology in designing a thermostable amylose-forming transglucosylase, J Biol Chem, 2022, 102074.

9. D. Hu, B.C. Hu, X.D. Hou, D. Zhang, Y.Q. Lei, Y.J. Rao*, M.C. Wu, Structure-Guided Regulation in the Enantioselectivity of an Epoxide Hydrolase for Producing Enantiomeric Monosubstituted Epoxides and Vicinal Diols via Kinetic Resolution, Org Lett, Accepted

10. W. Xu, D. W. Ni, X.D. Hou, T. Pijning, A. Guskov, Y.J. Rao, W.M. Mu, Crystal Structure of Levansucrase from the Gram-Negative Bacterium Brenneria Provides Insights into Its Product Size Specificity, J Agric Food Chem, 2022, 70(16), 5095-5105

11. Y.J. Yan, Z.Y. YU, W. Zhong, X.D. Hou, Q.Q. Tao, M.H. Cao, L. Wang, X.F. Cai, Y.J. Rao, S.X. Huang, Characterization of Multifunctional and Non-stereoselective Oxidoreductase RubE7/IstO, Expanding the Functional Diversity of the Flavoenzyme Superfamily, Angew, 2022, Accepted

12. H. Huang, X.D. Hou, R.R. Xu, Z.W. Deng, Y. Wang, G.C. Du, Y.J. Rao*, J. Chen, Z. Kang, Structure and cleavage pattern of a hyaluronate 3-glycanohydrolase in the glycoside hydrolase 79 family, Carbohyd Polym, 277 (2022).

13. Y.F. Liu, G.C. Xu, J.Y. Zhou, J. Ni, L. Zhang, X.D. Hou, D.J. Yin, Y.J. Rao*, Y.L. Zhao*, Y. Ni*, Structure-Guided Engineering of D-Carbamoylase Reveals a Key Loop at Substrate Entrance Tunnel, ACS Catal, 10 (2020) 12393-12402.