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当前位置: 首页   >  课题组新闻   >  研究生闫艳等在《环境昆虫学报》上发表“访花昆虫野蚜蝇线粒体基因组结构分析”
研究生闫艳等在《环境昆虫学报》上发表“访花昆虫野蚜蝇线粒体基因组结构分析”
发布时间:2021-07-05

中文摘要:

    野蚜蝇Syrphus torvus(Osten-Sacken)成虫具访花习性,是重要的传粉昆虫;幼虫捕食蚜虫,是蚜虫重要天敌之一。本研究通过高通量测序、组装拼接获得了完整的野蚜蝇线粒体基因组全序列(GenBank登录号: MW074962),其序列全长为16 444 bp,包含13个蛋白编码基因(PCGs)、22个tRNAs、2个rRNAs和D-loop区。野蚜蝇线粒体全序列A+T含量为80.6%,G+C含量为19.4%,表现出明显的A+T偏斜。除了COXI、ATP8、ATP6和ND1的起始密码子为TTG,其它9个PCGs以ATN为起始密码子;COXI的终止密码子为不完整的T--,ATP6为TAG,其余11个PCGs的终止子为TAA。22个tRNAs的二级结构均为典型的三叶草结构,并预测了野蚜蝇rRNAs的二级结构。野蚜蝇D-loop区存在重复序列和茎环结构,22种蚜蝇科基因间隔区与重叠区具有6个保守区域。基于24个物种(22个蚜蝇科和2个缟蝇科)的PCGs序列,通过贝叶斯法(BI)和最大似然法(ML)构建系统发育树,结果显示,野蚜蝇和黑足蚜蝇Syrphus vitripennis互为姊妹种,支持了蚜蝇亚科Syrphinae的单系性,两种拓扑结构中管蚜蝇亚科Eristalinae均没有聚为一支。该研究结果丰富了蚜蝇科线粒体基因组学基本数据,为进一步深化蚜蝇科系统发育关系讨论提供了参考。


英文摘要:

    Syrphus torvus (Osten-Sacken) belongs to the family Syrphidae of Diptera. The adults are flower visitors, are important pollinating insects; the larvae feed on aphids, are one of the important natural enemies of aphids. In this study, the genome of S. torvus was obtained by high-throughput sequencing and the complete mitochondrial genome (mitogenome) was assembed (GenBank accession No. MW074962). The length of complete mitogenome was 16 444 bp, including 13 protein coding genes (PCGs), 22 tRNAs, 2 rRNAs and D-loop. There were 80.6% A+T content and 19.4% G+C content in this complete mitogenome of S. torvus,showed significant A+T bias. All of PCGs, except COXI, ATP8, ATP6, and ND1 start codons were TTG, all start codon were ATN; all stop codons were TAA but COXI was incomplete T-- and ATP6 was TAG. All tRNAs were the typical cloverleaf secondary structure. The rRNAs secondary structures of S. torvus were predicted. There were four repeats and three stem-loop structures in the D-loop of S. torvus. Six conserved regions were possessed in Intergenic nucleotides and overlap of 22 Syrphidae. The phylogenetic tree was built by PCGs of 24 species  (22 Syrphidae and 2 Lauxaniidae) using methods of Bayesin (BI) and Maximum Likelihood (ML). The results showed that S. torvus and Syrphus vitripennis were sister group, support monophyly of Syrphinae, Eristalinae not gather one clade in two topology structures. The results enriched the basic data of genomics of Syrphidae mitochondrial and provide a reference for further discussion of phylogenetic relationship of Syrphidae.


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