The outbreak of SARS-coronavirus 2 (SARS-CoV2) has become a global health emergency.

Although enormous efforts have been made, there is still no effective treatment against the new

virus. Herein, a TiO2 supported single-atom nanozyme containing atomically dispersed Ag

atoms (Ag-TiO2 SAN) is designed to serve as a highly efficient antiviral nanomaterial.

Compared with troditional nano-TiO2 and Ag, Ag-TiO2 SAN exhibits higher adsorption

(99.65%) of SARS-CoV2 pseudovirus. This adsorption ability is due to the interaction between

SAN and receptor binding domain (RBD) of spike 1 protein of SARS-CoV2. Theoretical

calculation and experimental evidience indicate that the Ag atoms of SAN strongly bind to

cysteine and asparagine, which are the most abundant amino acids on the surface of spike 1

RBD. After binding to the virus, the SAN/virus complex is typically phagocytosed by

macrophages and colocalized with lysosomes. Interestingly, Ag-TiO2 SAN possesses high

peroxidase-like activity responsible for reactive oxygen species production under acid

conditions. The highly acidic microenvironment of lysosomes could favor oxygen reduction

reaction process to eliminate the virus. With hACE2 transgenic mice, Ag-TiO2 SAN showed

efficient anti-SARS-CoV2 pseudovirus activity. In conclusion, Ag-TiO2 SAN is a promising

nanomaterial to achieve effective antiviral effects for SARS-CoV2.