近日，2020级博士生梁慧慧在SCI一区期刊Biosensors and Bioelectronics (IF=12.6) 发表基于杂多酸COF催化抗坏血酸信号放大策略的神经元特异性烯醇化酶免疫传感“Immunosensing of neuron-specific enolase based on signal amplification strategies via catalysis of ascorbic acid by heteropolysate COF”。

原文链接：https://doi.org/10.1016/j.bios.2023.115593

Abstract

In view of the importance of quantification of neuron-specific enolase (NSE), an electrochemical NSE immunosensor was developed. The sandwich voltammetric immunosensor utilized vinyl-functionalized crystalline covalent organic framework (COF_TAPT-Dva) modified electrode to load lots of Ab₁ via thiol-ene “click” reaction as matrix. A crystalline cationic EB-COF:Br was used to load Au nanoparticles (AuNPs) and H3[PMo₁₂O₄₀] (PMo₁₂) as immunoprobe. The AuNPs with the size of about 30 nm were firstly grown on EB-COF:Br and then a large number of electroactive PMo₁₂ were uniformly assembled on AuNPs/EB-COF:Br via ion exchanging reaction. The AuNPs not only facilitated the bonding of Ab₂ based on Au–S bond, but also improved performance of Ab₂/AuNPs/EB-COF:PMo₁₂ immunoprobe. The sensitivity of sandwich electrochemical immunosensor could be primarily amplified based on loaded abundant PMo₁₂. Secondary sensitivity amplification of immunosensor could be achieved by using PMo₁₂ to catalyze ascorbic acid. The linear range of sandwich voltammetric immunosensor based on current change of differential pulse voltammetry is 500 ± 36 fg mL⁻¹ - 100 ± 8 ng mL⁻¹. Thanks to the dual sensitivity amplification strategy, the sensitivity is as high as 54.06 ± 3.2 μA cm⁻²/lg(cNSE/ng mL⁻¹), and the detection limit is as low as 166 ± 10.8 fg mL⁻¹. It proves that it is completely feasible to amplify sensitivity of sandwich voltammetric immunosensors using polyoxometalate-COF and its catalytic substrate.