Microbiome science is a multi-disciplinary field, but classical microbiologists are needed to ensure advances are grounded in our understanding of basic microbiological concepts.

Metabolic engineering guided by laboratory evolution enables upcycling of nylon by Pseudomonas putida.

ARF4 GTPase activity is needed for vesicular trafficking for multiple RNA viruses. Blocking ARF4 using specific peptides redirects viral progeny to lysosomal degradation and decreases influenza and Zika virus infection in mice.

Hexa-acylated lipopolysaccharides from the gut microbiota are associated with efficacy in patients with cancer receiving anti-PD-1 and stimulate host immune activation for enhanced anti-tumour immunity in mouse models.

Intestinal crypt-resident Acinetobacter radioresistens secretes indole acetic acid which activates an Ahr-Wnt-β-catenin-EphB2 signalling axis to promote intestinal stem cell homeostasis and prevent tumour initiation and colorectal cancer.

ARF4 GTPase activity is needed for vesicular trafficking for multiple RNA viruses. Blocking ARF4 using specific peptides redirects viral progeny to lysosomal degradation and decreases influenza and Zika virus infection in mice.

Akkermansia muciniphila can promote intestinal pathogen infection in mice via microbial metabolite succinate production, but the expansion of A. muciniphila is strictly controlled by ILC3s through the regulation of intestinal galactosylation.

Tecovirimat forces dimerization of the poxvirus phospholipase F13 blocking virus spread. Clinical monkeypox virus strains resistant to tecovirimat show mutations in the F13 dimer interface.

In a randomized controlled trial, the effect of sulforaphane (provided as a broccoli sprout extract) on fasting blood glucose in individuals with prediabetes was tested, in the context of gut microbiota composition and function.

Quantitative flow virometry assay to measure the shape of viral particles under various conditions reveals that influenza virus infections dynamically tune shape distribution of progeny particles depending on viral efficiency.

We provide guidance on the planning, execution and description of statistical analyses in microbiome studies.

Determining whether human organs house a resident microbiome is challenging and the findings can be controversial. However, forging collaborations and careful study design can help overcome these issues.

Microbiome science is a multi-disciplinary field, but classical microbiologists are needed to ensure advances are grounded in our understanding of basic microbiological concepts.

Global food insecurity is a growing crisis, but microbes could offer an effective tool for sustainable agriculture.

The Oropouche virus has long been overlooked, but following a recent global expansion the virologist Marta Giovanetti argues for a One Health strategy to address this emergent public health threat.