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个人简介

钟涛教授主要以斑马鱼和小鼠为模式动物,结合人类干细胞,利用遗传学、发育生物学、化学生物学和分子生物学的方法研究心脏和血管的分化形成与再生的生物学机制。在国际科学界,第一次提出动脉血管内皮细胞在血液循环之前就已获得分子特征的概念,发现和克隆心血管发育的核心转录因子Grl/Hey2家族及其上游关键信号NOTCH通路。率先揭示了前列腺素(PGE2)信号及其转运蛋白(ABCC)调控细胞纤毛生长与心脏及多种器官形态发育的新颖生物学机制。以第一作者或通讯作者在包括Cell,Nature, Science, Nature Cell Biology等国际一流学术杂志上发表了80多篇文章。关于血管内皮细胞分化的研究成果被美国大学经典教科书《发育生物学》(第十版)采用(教科书级别重大成果)。 教育经历 1995年7月-2000年8月:哈佛大学医学院,麻省理工学院 博士后 1990年1月-1995年6月:美国纽约州立大学,冷泉港研究所 博士 1987年7月-1989年12月:复旦大学医学院(原上海医科大学) 硕士 1982年9月-1987年6月:复旦大学医学院(原上海医科大学) 本科

研究领域

利用基因编辑工具CRISPR/Cas9构建转基因、突变体斑马鱼以及开发更高效的单碱基编辑工具 细胞纤毛与胚胎及心脏生长发育机制研究 探索心脏发育及再生机制 研究造血干细胞增殖和分化的分子及细胞机制

近期论文

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Xue N, Liu X, Zhang D, Wu Y, Zhong Y, Wang J, Fan W, Jiang H, Zhu B, Ge X, Gonzalez RVL, Chen L, Zhang S, She P, Zhong Z, Sun J, Chen X, Wang L, Gu Z, Zhu P, Liu M, Li D, Zhong TP*, Zhang X*. 2023. Improving adenine and dual base editors through introduction of TadA-8e and Rad51DBD. Nature Communications. 14(1):1224. *Correspondent author Liu X, Yu T, Tan X, Jin D, Yang W, Zhang J, Dai L, He Z, Li D, Zhang Y, Liao S, Zhao J, Zhong TP*, Liu C*. 2023. Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2. Elife. 12:e81438. *Correspondent author Wu J, Li J, Chen K, Liu G, Zhou Y, Chen W, Zhu X, Ni TT, Zhang B, Jin D, Li D, Kang L, Wu Y, Zhu P, Xie P, Zhong TP. 2023. Atf7ip and Setdb1 interaction orchestrates the hematopoietic stem and progenitor cell state with diverse lineage differentiation. Proc Natl Acad Sci. 120(1):e2209062120. Li D, Sun J, Zhong TP. 2022. Wnt Signaling in Heart Development and Regeneration. Curr Cardiol Rep. 24(10):1425-1438. Jin D, Zhong TP. 2022. Prostaglandin signaling in ciliogenesis and development. J Cell Physiol. 237(6):2632-2643. Liu, X.X., Pu, W.J., He, L.J., Li, Y., Zhao, H., Li, Y., Liu, K., Huang, X.Z., Weng, W.D., Wang, Q.D., Shen, L.H., Tao P. Zhong, Sun, K., Reza Ardehali, He, B., Zhou, B. 2021. Cell proliferation fate mapping reveals regional cardiomyocyte cell-cycle activity in subendocardial muscle of left ventricle. Nature Communications. 12(1):5784. Cai Y, Wang J, Jin D, Liu Q, Chen X, Pan L, Li Y, Wang X, Qian F, Wang J, Zhong TP*, Wang S*. 2021. Dhx15 regulates zebrafish definitive hematopoiesis through the unfolded protein response pathway. Cancer Sci. 112(9):3884-3894. *Correspondent author Wei G, Sun H, Dong K, Hu L, Wang Q, Zhuang Q, Zhu Y, Zhang X, Shao Y, Tang H, Li Z, Chen S, Lu J, Wang Y, Gan X, Zhong TP, Gui D, Hu X, Wang L, Liu J. 2021. The thermogenic activity of adjacent adipocytes fuels the progression of ccRCC and compromises anti-tumor therapeutic efficacy. Cell Metab. 33(10):2021-2039.e8. Peilu She, Huifang Zhang, Xian.gwen Peng, Jianjian Sun, Bangjun Gao, Yating Zhou, Xuejiao Zhu, Xueli Hu, Kaa Seng Lai, Jiemin Wong, Bin Zhou, Linhui Wang, Tao P. Zhong. 2020. The Gridlock/Hey2 Transcriptional Repressor Impedes Vertebrate Heart Regeneration by Restricting Expression of Lysine Methyltransferase. Development. 147(18):dev190678. Peng X, Lai KS, She P, Kang J, Wang T, Li G, Zhou Y, Sun J, Jin D, Xu X, Liao L, Liu J, Lee E, Poss KD, Zhong TP. 2021. Induction of Wnt signaling antagonists and p21-activated kinase enhances cardiomyocyte proliferation during zebrafish heart regeneration. J Mol Cell Biol. 13(1):41-58. Fang Y, Lai KS, She P, Sun J, Tao W, Zhong TP. 2020. Tbx20 Induction Promotes Zebrafish Heart Regeneration by Inducing Cardiomyocyte Dedifferentiation and Endocardial Expansion. Front Cell Dev Biol. 8:738. Sun Y, Zhang B, Luo L, Shi DL, Wang H, Cui Z, Huang H, Cao Y, Shu X, Zhang W, Zhou J, Li Y, Du J, Zhao Q, Chen J, Zhong H, Zhong TP, Li L, Xiong JW, Peng J, Xiao W, Zhang J, Yao J, Yin Z, Mo X, Peng G, Zhu J, Chen Y, Zhou Y, Liu D, Pan W, Zhang Y, Ruan H, Liu F, Zhu Z, Meng A; ZAKOC Consortium. 2019. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9. Genome Res. 30(1):118–26. Zhao W, Cao L, Ying H, Zhang W, Li D, Zhu X, Xue W, Wu S, Cao M, Fu C, Qi H, Hao Y, Tang YC, Qin J, Zhong TP, Lin X, Yu L, Li X, Li L, Wu D, Pan W. 2019. Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition. Cell Res. 29(11):895-910. Xie S, Fu W, Yu G, Hu X, Lai KS, Peng X, Zhou Y, Zhu X, Christov P, Sawyer L, Ni TT, Sulikowski GA, Yang Z, Lee E, Zeng C, Wang WE, Zhong TP. 2019. Discovering small molecules as Wnt inhibitors that promote heart regeneration and injury repair. J Mol Cell Biol. 12(1):42-54 Li G, Jin D, Zhong TP. 2019. Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Development. Frontier in Mol Neurosci. 12:126 Li W, Jin D, Zhong TP. 2019. Photoreceptor cell development requires prostaglandin signaling in the zebrafish retina. Biochem Biophys Res Commun.510(2):230-235 Zhang R, He J, Wang X, Pan W, Cao Y, Zu Y, Jiang Q, Du J, Zhong TP. 2019. Report of the Fifth Zebrafish Research Conference. Zebrafish.16(1):128-134. Xinyu Yang, Jiani Li, Yabo Fang, Zhen Zhang, Daqing Jin, Xingdong Chen, YongtaoGuan, Yan Zhao, Mengqi Li, Linchun Huan, Thomas A. Kent, Jing-fei Dong, Rongcai Jiang, Shuyuan Yang, Li Jin, Jianning Zhang, Zhong TP+, Fuli Yu+. 2018. Rho Guanine Nucleotide Exchange Factor ARHGEF17 is a risk gene for Intracranial Aneurysms. Circulation: Genomic and Precision Medicine. 11(7):e002099. +Correspondent author Daqing Jin, Diqi Zhu, Yabo Fang, Yiwei Chen, Gaihong Yu, Weijun Pan, Dong Liu, Fen Li, Zhong TP. 2017. Vegfa signaling regulates diverse artery/vein formation in vertebrate vasculatures. J Genet Genomics. 44(10):483-492 Xueying Tian, Yan Li, Lingjuan He, Hui Zhang, Xiuzhen Huang, Qiaozhen Liu, WenjuanPu, Libo Zhang, Yi Li, Huan Zhao, Zhifu Wang, Jianhong Zhu, Yu Nie, Shengshou Hu, David Sedmera, Zhong TP, Sean Wu, William Pu, Robert Anderson, Bin Zhou, Ying Yu, Li Zhang, Yan Yan, Zengyong Qiao, and Qing-Dong Wang. 2017. Identification of a hybrid myocardial zone in the mammalian heart after birth. Nature Communications. 8(1):87. Zeng S., Zhong TP. 2017. Cardioascular Research in Zebrafish. Encyclopedia of Cardiovascular Research and Medicine. vol 4. p759-770. Vasan R, Sawyer D ed. Oxford: Elsevier,inc. Peiyun Liu, Zhong TP. 2017. MAPK/ERK signalling is required for zebrafish cardiac regeneration. Biotechnol Lett.39(7):1069-77 Diqi Zhu, Yabo Fang, Kun Gao, Jie Shen, Zhong TP+ and Fen Li+. 2017. Vegfa Impacts Early Myocardium Development. Int J Mol Sci. 18(2). +Correspondent author Da Wo, Jinhui Peng, Dan-ni Ren, Liman Qiu, Jinxiao Chen, Ye Zhu, Yingjing Yan,Hongwei Yan, Jian Wu, En Ma, Zhong TP, Yi-Han Chen, Zhong-Min Liu, Shangfeng Liu, Luoquan Ao, Zhenping Liu, Cizhong Jiang, Jun Peng, Yunzeng Zou, Qirong Qian, Weidong Zhu. 2016. Opposing Roles of Wnt Inhibitors IGFBP-4 and Dkk1 in Cardiac Ischemia by Differential Targeting of LRP5/6 and β–catenin. Circulation. 134(24):1991-2007. Sun Jianjian, Zhihan Wu and Zhong TP. 2016. Cila Function in Cell Signaling and Organ Development. Scientia Sinica Vitae. 46,4:354-62 Peng XW, He QZ, Li GB, Ma JM, Zhong TP. 2016. Rac1-PAK2 pathway is essential for heart regeneration. Biochem Biophys Res Commun. 472:637-642. Li Q, Yang H, Zhong TP. 2015. Regeneration across Metazoan Phylogeny: Lessons from Model Organisms. J Genet Genomics. 42:57-70. Nan Wu, Xuan Ming, Jianqiu Xiao, Zhihong Wu, Xiaoli Chen, Marwan Shinawi, Yiping Shen, Guangju Yu, Jiaqi Liu, Hua Xie, Zoran S. Gucev, Sen Liu, Nan Yang, Hussam Al-Kateb, Jun Chen, Jian Zhang, Natalie Hauser, Ting Zhang, Velibor Tasic, Pengfei Liu, Xinlin Su, Xuedong Pan, Chunyu Liu, Liwen Wang, Joseph Shen, Jianxiong Shen, Yulin Chen, Ting Zhang, Jianguo Zhang, Kwong Wai Choy, Jun Wang, Qiqi Wang, Shugang Li, Weichen Zhou, Jin Guo, Yipeng Wang, Cheng Zhang, Hong Zhao, Yu An, Yu Zhao, Jiucun Wang, Zhenlei Liu, Yuzhi Zuo, V. Reid Sutton, Hongyan Wang, Yue Ming, Shashikant Kulkarni, Zhong TP, Sau Wai Cheung,Xue Zhang, Li Jin, James R. Lupski, Guixing Qiu, and Feng Zhang. 2015. Compound Inheritance of TBX6 Rare Null Variants and a Common Hypomorphic Allele in Congenital Scoliosis. New England Journal of Medicine. 372(4):341-50. Jin D, Liu PY, Zhong TP. 2015. Prostaglandin Signaling in Ciliogenesis during Development. Cell Cycle. 14 (1):1-2. Tian X, Hu T, Zhang H, He L, Huang X, Liu Q, Yu W, He L, Yang Z, Yan Y, YangX, Zhong TP, Pu WT, Zhou B. 2014. De Novo formation of a distinct coronary vascular population in neonatal heart. Science. 345 (6192): 90-4. Du X, Shi H, Li J, Dong Y, Liang J, Ye J, Kong S, Zhang S, Zhong TP, Yuan Z, Xu T, Zhuang Y, Zheng B, Geng JG, Tao W. 2014. Mst1/Mst2 regulate development and function of regulatory T cells through modulation of Foxo1/Foxo3 stability in autoimmune disease. J Immunol. 192(4):1525-35. Jin D, Ni TT, Sun J, Wan H, Amack JD, Yu G, Fleming J, Chiang C, Li W, Papierniak A, Cheepala S, Conseil G, Cole SP, Zhou B, Drummond IA, Schuetz JD, Malicki J, Zhong TP. 2014. Prostaglandin signaling regulates ciliogenesis and heart development by modulating intraflagellar transport. Nature Cell Biology.16 (9):841-51. (Article & Cover story; Previews by Barbry P & Zaragosi L E : An ABC of ciliogenesis. 2014. Nature Cell Biololgy. 16(9):826-7). Dai X, She P, Chi F, Feng Y, Liu H, Jin D, Zhao Y, Guo X, Jiang D, Guan KL, Zhong TP, Zhao B. 2013. Phosphorylation of angiomotin by Lats1/2 kinases inhibits F-actin binding, cell migration and angiogenesis. J Biol Chem. 288 (47):34041-51. Sun YP, Dong ZQ, Jin TH, Ang KK, Huang M, Haston KM, Peng J, Zhong TP, Finkbeiner, S, Weiss WA, Jan LY, Guo S. 2013. Imaging-based chemical screening reveals activity-dependent neural differentiation of pluripotent stem cells. Elife. e00508 (HHMI Press) Cheepala SB, Bao J, Nachagari D, Sun D, Wang Y, Zhong TP, Naren AP, Zheng J, Schuetz JD. 2013. Crucial role for phylogenetically conserved cytoplasmic loop3 inABCC4 expression. J Biol Chem. 288(31):22207-18. Tian X, Hu T, Zhang H, He L, Huang X, Liu Q, Yu W, He L, Yang Z, Zhang Z, Zhong TP, Yang X, Yang Z, Yan Y, Baldini A, Sun Y, Lu J, Schwartz RJ, Evans SM, Gittenberger-de Groot AC, Red-Horse K, Zhou B. 2013. Subepicardial endothelial cells invade the embryonic ventricle wall to form coronary arteries. Cell Research. 14 (5):371-45 (NaturePress) Xiang Y, Cho PH, Zeng X, Zheng XJ, Jessen JR, Zhong TP, Xu XL. 2013. Trapping Cardiac Recessive Mutants via Expression-based Insertional Mutagenesis Screening. Circulation Research. 112 (4):606-17 Jin DQ, Li Q and Zhong TP. 2013. Chemical Genetics in Cardiomyocyte Generation. Chapter III in “Chemical Biology in Regenerative Medicine: Bridging Stem Cells and Future Therapies”. Wiley Publication, inc. USA Ni TT, Lu J, Zhu M, Maddison LA, Boyd KL, Huskey L, Ju B, Hesselson D, Zhong TP, Page- McCaw PS, Stainier DY, Chen W. 2012. Conditional control of gene function by an invertible gene trap in zebrafish. Proc Natl Acad Sci. 109:15389-94. Ni TT, Rellinger E, Williams C, Stephens L, Hu JY, Kim K, Marnett L, Heaton W, Hatzopoulos A, Zhong TP.2011. Discovering small molecules that promote cardiomyocyte generation via modulating Wnt signaling. Chemistry & Biology. 8:1658-68 (CellPress). (Previews by Ruey J. Yeh: A Wnt Inhibitors with a Twist. Chemistry & Biology. 18:1518-1520, 2011; Lab Reports, JAMA 307:5, 446, 2012) Chopra SS, Watanabe V, Yang T, Stroud DM, Burns CG, Wells S, Zhong TP+, Roden DM. 2010. A non-electrogenic requirement for voltage-gated sodium channels in zebrafish heart development. Circulation Research. 106:1342-50. +Correspondent author Williams C, Kim SH, Ni TT, Mitchell L, Ro HJ, Penn J, Baldwin HS, Solnica-Krezel L, Zhong TP.2010. Hedgehog signaling induces arterial endothelial cell formation by repressing venous cell fate. Developmental Biology. 341,196-204 Jin D, Ni TT, Hou J, Rellinger E, Zhong TP. 2009. Promoter analysis of ventricular myosin heavy chain (vmhc) in zebrafish embryos. Developmental Dynamics. 238,1760-1767 Zeng X, Zheng XJ, Xiang Y, Cho PH, Jessen JR, Zhong TP, Solnica-Krezel L and Brown AL. 2009. Phospholipase D1 is required for angiogenesis of intersegmental blood vessels in zebrafish. Developmental Biology. 328,363-76 Ni TT, William L, Shyr Y, Zhong TP. 2008. Use of normalization methods for analysis of microarrays containing ahigh degree of gene effects. BMC Bioinformatics. 9, 505. Qu XH, Jia HB, Garrity DM, Tompkins K, Batts L, Appel B, Zhong TP+, Baldwin S. 2008. Ndrg4is required for normal myocyte proliferation during early cardiac development in zebrafish. Developmental Biology. 317, 486-96. +Correspondent author Wang YX, Qian LX, Liu D, Yao LL, Gui YH, Zhong TP. Song HY. 2007. Bone morphogenetic protein-2 acts upstream of myocyte-specific enhancer factor 2a to control embryonic cardiac contractility. Cardiovascular Research. 74 (2):290-303. Chopra S, Hiroshi Watanable, Zhong TP+, Roden D. 2007. Molecular cloning and analysis of zebrafish voltage-gated sodium channel b-subunits: Implication for the evolution of electrical signaling in vertebrates. BMC Evolution Biology. 7, 113-7. +Correspondent author. Chopra S and Zhong TP. 2007. Vascular Development in Zebrafish. Endothelial Biomedicine. 1107-1127. Cambridge: Cambridge University Press, UK. Jia H, King I, Chopra S, Wan H, Ni T, Jiang C, Guan X, Well S, Srivastava D and Zhong TP. 2007. Vertebrate heart growth is regulated by functional antagonism between Gridlock and Gata5. Proc Natl Acad Sci. 104,14008-14013. Campbell WA, Yang H, Zetterberg H, Baulac S, Sears JA, Liu T, Wong STC, Zhong TP, XiaW. 2006. Zebrafish lacking Alzheimer presenilin enhancer 2 (Pen-2) demonstrate excessive p53 dependent apoptosis and neuronal loss. J Neurochemistry. 96,1423-40. Rutenberg JB, Fischer A., Jia HB, Gessler M., Zhong TP and Mercola M. 2006. Developmental patterning of the cardiac atrioventricular canal by Notch and Hairy-related transcription factors. Development. 133,4381-90. Wang Y, Zhong TP, Qian L, Dong Y, Jiang Q, Tan L, Song H. 2005. Wortmannin induces zebrafish cardia bifida through a mechanism independent of phosphoinositide 3-kinase and myocisn light chain kinase. Biochem Biophys Res Commun. 331 (1):303-8 Zhang L, Zhong TP, Wang Y, Jiang Q, Song H, Gui Y. 2005. TBX1, a DiGeorge syndrome candidate gene, is inhibited by retinoic acid. Int. J. Developmental Biology. 50:55-61 Wang Y, Qian L, Zhang Y, Jiang Q, Dong Y, Liu X, Yang X, Zhong TP+, Song H. 2005. Requirements of myocyte-specific enhancer factor 2A in zebrafish cardiac contractility. FEBS. 579, 4843-50. +Correspondent author Zhong TP. 2005. Zebrafish Genetics and Formation of Embryonic Vasculature. Current Topics in Development Biology. 71,53-81. Peterson R, Shaw SY, Peterson TA, Milan DJ, Zhong Tao P, Schreiber S, Mac Rae C and Fishman, MC. 2004. Chemical suppression of a genetic mutation in a zebrafish model of aortic coarctation. Nature Biotechnology. 22, 593-599. Xu X, Miller S, Zhong TP, Mohideen M, Crossley DA, Burggren WW and Fishman MC. 2002. Cardiomyopathy in zebrafish due to mutation in an alternatively spliced exon of Titin. Nature Genetics. 30, 205-209. Zhong TP, Childes S, Leu J, Fishman MC. 2001. The Gridlock signaling pathway fashions the first embryonic artery. Nature. 414, 216-220. (Comment by George D. Yancopoulos: Gridlock in the blood. Nature. 404, 163-164) Zhong TP, Rosenberg M, Mohideen M, Weinstein B, Fishman MC. 2000. Gridlock, an HLH gene required for assembly of the aorta in zebrafish. Science. 287, 1820-1824. Amemiya C, Zhong TP, Silverman G, Fishman MC, Zon L. 1999. In the zebrafish: Genetics and Genomics. Methods in Cell Biol. H.W. Detrich, III, M. Westerfield, L.I. Zon, Eds. (Academic Press, San Diego) pp.236. Zhong TP, Kaphingst K, Akella U, Haldi M, Lander E and Fishman MC. 1998. Zebrafish genomic library in yeast artificial chromosome. Genomics. 48, 136-138. Zhong TP, Luke M and Arndt K. 1996. Transcriptional regulation of the yeast DnaJ molecule SIS1 gene. J. Biological Chemistry. 271, 1349-1356. Zhong TP and Arndt K. 1993. Yeast SIS1 protein, a DnaJ homologue, is required for the Initiation of translation. Cell. 73, 1175-1186. Sarabia M, Sutton A, Zhong TP and Arndt K. 1992. SIT4 protein phosphatase is required for the normal accumulation of SWI4, CLN1, CLN2, and HCS26 RNAs during Late G1. Genes & Development. 6, 2417-2428.

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