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个人简介

1992-1996 清华大学生物科学与技术系,学士 1996-2001 清华大学生物科学与技术系,博士 2001-2006 美国劳伦斯伯克利国家实验室生命科学部,博士后 2006-2008 美国劳伦斯伯克利国家实验室生命科学部,研究科学家 2009-2011 美国耶鲁大学分子生物物理与生物化学系,Tenure-Track助理教授 2010.12-至今 清华大学生命科学学院,教授、博导 2016.04-2021.04 清华大学生命科学学院院长

研究领域

利用冷冻电子显微学研究生物大分子复合体的结构与分子机理。主要研究方向如下: 1. 更高效、更高分辨率的冷冻电镜方法学开发 2. 核酸质量控制的分子机制和调控 3. 细胞骨架和生物膜系统的协调机制

近期论文

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You X, Zhang X, Cheng J, Xiao YN, Ma JF, Sun S, Zhang XZ*, Wang HW*, Sui SF*. (2023) In situ structure of the red algal phycobilisome–PSII–PSI–LHC megacomplex. Nature, 616(7955):199-206. Zheng LM, Liu N, Gao XY, Zhu WQ, Liu K, Wu C, Yan R, Zhang JC, Gao X, Yao YT, Deng B, Xu J, Lu Y, Liu, ZM, Li MS, Wei XD*, Wang HW*, Peng HL*. (2023) Uniform thin ice on ultraflat graphene for high-resolution cryo-EM. Nat. Methods, 20(1):123-130. Liu N, Wang HW*. (2022) Better cryo-EM specimen preparation: How to deal with the air-water interface? J. Mol. Biol., doi: 10.1016/j.jmb.2022.167926. Online ahead of print. Lu Y, Liu N*, Liu Y, Zheng LM, Yang JH, Wang J, Jia X, Zi QR, Peng HL*, Rao Y*, Wang HW*. (2022) Functionalized graphene grids with various charges for single-particle cryo-EM. Nat. Commun., 13(1):6718. Yan A, Xiong J, Zhu J, Li X, Xu S, Feng X, Ke X, Wang Z, Chen Y, Wang HW, Zhang MQ*, Kee K*. (2022) DAZL regulates proliferation of human primordial germ cells by direct binding to precursor miRNAs and enhances DICER processing activity. Nucleic Acids. Res., 50(19):11255-11272. Su S, Wang J, Deng T, Yuan X, He J, Liu N, Li XM, Huang Y, Wang HW*, Ma JB*. (2022) Structural insights into dsRNA processing by Drosophila Dicer-2-Loqs-PD. Nature, 607(7918):399-406. Huang Y, Zhang X, Wang HW, Yu L*. (2022) Assembly of Tetraspanin-enriched macrodomains contains membrane damage to facilitate repair. Nat. Cell Biol., 24(6):825-832. Wang X, Hu CX, Ye W, Wang J, Dong X, Xu J, Li X, Zhang M, Lu H, Zhang F, Wu W, Dai S, Wang HW*, Chen Z*. (2022) Structure of Rift Valley fever virus RNA-dependent RNA polymerase. J. Virol., 96(3):e0171321. Xu JF, Zhao LY, Peng SJ, Chu HY, Liang R, Tian M, Connell PP, Li G, Chen CL*, Wang HW*. (2021) Mechanisms of distinctive mismatch tolerance between Rad51 and Dmc1 in homologous recombination. Nucleic Acids Res., 49(22): 13135–13149. Zhang J, Jia K, Huang Y, Wang Y, Liu N, Chen YN, Liu X, Liu X, Zhu Y, Zheng L, Chen H, Liang F, Zhang M, Duan X, Wang HW, Lin L*, Peng HL*, Liu ZF*. (2021) Hydrophilic, Clean Graphene for Cell Culture and Cryo-EM Imaging. Nano. Lett., 21(22):9587-9593. Chen J, Liu N, Huang Y, Wang Y, Sun Y, Wu Q, Li D, Gao S, Wang HW*, Huang N*, Qi X*, Wang XD*. (2021) Structure of PDE3A-SLFN12 complex and structure-based design for a potent apoptosis inducer of tumor cells. Nat. Commun., 12(1):6204. Zheng L, Liu N, Liu Y, Li N, Zhang J, Wang C, Zhu W, Chen Y, Ying D, Xu J, Yang Z, Gao X, Tang J, Wang X, Liang Z, Zou R, Li Y, Gao P, Wei X*, Wang HW*, Peng HL*. (2021) Atomically Thin Bilayer Janus Membranes for Cryo-electron Microscopy. ACS Nano, 15(10):16562-16571. Niu S, Wang J, Bai B, Wu L, Zheng A, Chen Q, Du P, Han P, Zhang Y, Jia Y, Qiao C, Qi J, Tian WX*, Wang HW*, Wang Q*, Gao GF*. (2021) Molecular basis of cross-species ACE2 interactions with SARS-CoV-2-like viruses of pangolin origin. EMBO J., 40(16):e107786. Nie J, Xie J, Liu S, Wu J, Liu C, Li J, Liu Y, Wang M, Zhao H, Zhang Y, Yao J, Chen L, Shen Y, Yang Y, Wang HW*, Wang Y*, Huang W*. (2021) Three epitope-distinct human antibodies from RenMab mice neutralize SARS-CoV-2 and cooperatively minimize the escape of mutants. Cell Discov., 7(1):53. Liu N, Zheng LM, Xu J, Wang J, Hu CX, Lan J, Zhang X, Zhang J, Xu K, Cheng H, Yang Z, Gao X, Wang X, Peng HL*, Chen YN*, Wang HW*. (2021) Reduced graphene oxide membrane as supporting film for high-resolution cryo-EM. Biophysics Reports, 7(3): 227-238. (Cover) Zhang S, Liu N, Wang HW, Lu Q, Shi W*, Wang X*. (2021) Sub-Nanometer Nanobelts Based on Titanium Dioxide/Zirconium Dioxide-Polyoxometalate Heterostructures. Adv. Mater., 33(23):e2100576. Xu J, Cui XY, Liu N, Chen YN*, Wang HW*. (2021) Structural engineering of graphene for high-resolution cryo-electron microscopy. SmartMat., 2:202–212 (Review). Xu K, Liu N, Xu J, Guo CL, Zhao LY, Wang HW, Zhang QF*. (2021) VRmol: an Integrative Web-Based Virtual Reality System to Explore Macromolecular Structure. Bioinformatics, 37(7):1029-1031. Yan R, Wang R, Ju B, Yu J, Zhang Y, Liu N, Wang J, Zhang Q, Chen P, Zhou B, Li Y, Shen Y, Zhang S, Tian L, Guo Y, Xia L, Zhong X, Cheng L, Ge X, Zhao J, Wang HW, Wang XQ, Zhang Z*, Zhang LQ* and Zhou Q*. (2021) Structural basis for bivalent binding and inhibition of SARS-CoV-2 infection by human potent neutralizing antibodies. Cell Res., 31(5):517-525. Liu K, Tan S, Niu S, Wang J, Wu L, Sun H, Zhang Y, Pan X, Qu X, Du P, Meng Y, Jia Y, Chen Q, Deng C, Yan J, Wang HW, Wang Q*, Qi J*, Gao G*. (2021) Cross-species recognition of SARS-CoV-2 to bat ACE2. Proc. Natl. Acad. Sci. USA, 118(1):e2020216118. Liu JL, Wang SB, Liu N, Yang DR, Wang HW*, Hu HS*, Zhuang J, Wang X*. (2020) Au-Polyoxometalates A-B-A-B Type Copolymer-Analogue Sub-1 nm Nanowires. Small, 17(4):e2006260. Liu N, Dong XL, Hu CX, Zeng J, Wang JW, Wang J, Wang HW*, Belfort M*. (2020) Exon and protein positioning in a pre-catalytic group II intron RNP primed for splicing. Nucleic Acids. Res., 48(19):11185-11198. Liu JL, Liu N, Wang HW*, Shi WX*, Zhuang J, Xun Wang*. (2020) Hybrid MoO3-Polyoxometallate Sub-1 nm Nanobelt Superstructures. J. Am. Chem. Soc., 142(41):17557-17563. Wu LL, Chen Q, Liu KF, Wang J, Han PC, Zhang YF, Hu Y, Meng YM, Pan XQ, Qiao CP, Tian SY, Du P, Song H, Shi WF, Qi JX, Wang HW*, Yan JH*, Gao GF, Wang QH*. (2020) Broad host range of SARS-CoV-2 and the molecular basis for SARS-CoV-2 binding to cat ACE2. Cell Discov., 6:68. Jin W, Wang J, Liu CP, Wang HW*, Xu RM*. (2020) Structural Basis for pri-miRNA Recognition by Drosha. Mol. Cell, 78(3): 423-+. Wang J, Song X, Zhang D, Chen X, Li X, Sun Y, Li C, Song Y, Ding Y, Ren R, Harrington EH, Hu LA, Zhong W, Xu C, Huang X, Wang HW*, Ma Y*. (2020) Cryo-EM structures of PAC1 receptor reveal ligand binding mechanism. Cell Research, 30(5): 436-455. Yuan D, Liu Z, Kaindl J, Maeda S, Zhao J, Sun X, Xu J, Gmeiner P*, Wang HW*, Kobilka BK*. (2020) Activation of the α2B adrenoceptor by the sedative sympatholytic dexmedetomidine. Nat. Chem. Biol., 16(5): 507-+. Shen W, Wang QW, Liu YN, Marchetto MC, Linker S, Lu SY, Chen Y, Liu C, Guo C, Xing Z, Shi W, Kelsoe JR, Alda M, Wang HW, Zhong Y, Sui SF, Zhao M, Yang Y, Mi S, Cao L, Gage FH*, Yao J*. (2020) Synaptotagmin-7 is a key factor for bipolar-like behavioral abnormalities in mice. Proc. Natl. Acad. Sci. USA, 117(8):4392-4399. Liu C, Ma J, Wang J, Wang HW, Zhang L*. (2020) Cryo-EM Structure of a Bacterial Lipid Transporter YebT. J. Mol. Biol., 432(4):1008-1019. Zheng L, Chen Y, Li N, Zhang J, Liu N, Liu J, Dang W, Deng B, Li Y, Gao X, Tan C, Yang Z, Xu S, Wang M, Yang H, Sun L, Cui Y, Wei X, Gao P*, Wang HW*, Peng H*. (2020) Robust ultraclean atomically thin membranes for atomic-resolution electron microscopy. Nat. Commun., 11(1):541. Liu JJ*, Wang HW*. (2020) Cryo-Electron Microscopy of Endogenous Yeast Exosomes. Methods Mol. Biol., 2062:401-415. (Book Chapter) Hu Y, Desimmie BA, Nguyen HC, Ziegler SJ, Cheng TC, Chen J, Wang J, Wang HW, Zhang K, Pathak VK*, Xiong Y*. (2019) Structural basis of antagonism of human APOBEC3F by HIV-1 Vif. Nat. Struct. Mol. Biol., 26:1176-1183. Carragher B, Cheng Y, Frost A, Glaeser RM*, Lander GC, Nogales E, Wang HW. (2019) Current outcomes when optimizing ‘standard’ sample preparation for single-particle cryo-EM. J. Microsc., 276:39-45. Dai A, Yu L*, Wang HW*. (2019) WHAMM initiates autolysosome tubulation by promoting actin polymerization on autolysosomes. Nat. Commun., 10: 3699. Wang HW*, Fan X. (2019) Challenges and opportunities in cryo-EM with phase plate. Curr. Opin. Struct. Biol., 58:175-182. (Review) Zhang R, Qu X, Zhang M, Jiang Y, Dai A, Zhao W, Cao D, Lan Y, Yu R, Wang HW, Huang S*. (2019) The Balance between Actin-Bundling Factors Controls Actin Architecture in Pollen Tubes. iScience, 16:162-176. Fan X, Wang J, Zhang X, Yang Z, Zhang JC, Zhao L, Peng HL, Lei J*, Wang HW*. (2019) Single particle cryo-EM reconstruction of 52 kDa streptavidin at 3.2 Angstrom resolution. Nat. Commun.,10:2386. Liu Y, Hu H, Wang J, Zhou Q, Wu P, Yan N, Wang HW, Wu JW, Sun L*. (2019) Cryo-EM structure of L-fucokinase/GDP-fucose pyrophosphorylase (FKP) in Bacteroides fragilis. Protein Cell, 10:365-369. Wang HW*. (2019) A Link between Intronic Polyadenylation and HR Maintenance Discovered. Biochemistry, 58:1835-1836. Wang J, Wang J, Hu M, Wu S, Qi J, Wang G, Han Z, Qi Y, Gao N, Wang HW*, Zhou JM*, Chai J*. (2019) Ligand-triggered allosteric ADP release primes a plant NLR complex. Science, 364:eaav5868. Wang J, Hu M, Wang J, Qi J, Han Z, Wang G, Qi Y, Wang HW*, Zhou JM*, Chai J*. (2019) Reconstitution and structure of a plant NLR resistosome conferring immunity. Science, 364:eaav5870. Liu N, Zhang J, Chen Y*, Liu C, Zhang X, Xu K, Wen J, Luo Z, Chen S, Gao P, Jia K, Liu Z, Peng H*, Wang HW*. (2019) Bioactive Functionalized Monolayer Graphene for High-Resolution Cryo-Electron Microscopy. J. Am. Chem. Soc., 141:4016-4025. Li XM, Lei JL*, and Wang HW*. (2018) The application of CorrSightTM in correlative light and electron microscopy of vitrified biological specimens. Biophys Rep., , 4(3):143-152. Liu Z, Wang J, Cheng H, Ke X, Sun L, Zhang Q, Wang HW*. (2018) Cryo-EM Structure of Human Dicer and Its Complexes with a Pre-miRNA Substrate. Cell, 173:1191-1203. Chen X, Liu M, Tian Y, Li J, Qi Y, Zhao D, Wu Z, Huang M, Wong CC, Wang HW, Wang J, Yang H*, Xu Y*. (2018) Cryo-EM structure of human mTOR complex 2. Cell Research, 28:518-528. Zhao L, Xu J, Zhao W, Sung P, Wang HW*. (2018) Determining the RAD51-DNA Nucleoprotein Filament Structure and Function by Cryo-Electron Microscopy. Methods Enzymol., 600:179-199. Rao Q, Liu M, Tian Y, Wu Z, Hao Y, Song L, Qin Z, Ding C, Wang HW*, Wang J*, Xu Y*. (2018) Cryo-EM structure of human ATR-ATRIP complex. Cell Research,28:143-156. Mei K, Li Y, Wang S, Shao G, Wang J, Ding Y, Luo G, Yue P, Liu JJ, Wang X, Dong MQ, Wang HW*, Guo W*. (2018) Cryo-EM structure of the exocyst complex. Nat. Struct. Mol. Biol., 25:139-146. (Cover)

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