林章凛 - 清华大学 - 化学工程系

个人简介

Education and Work Experience 1990 B.S., Chemical Engineering (with a minor in economics), Tsinghua University 1993 M.S., Biochemical Engineering, University of Maryland, Baltimore 1996 Ph.D., Molecular and Cell Biology, University of Maryland 1996 – 1999 Postdoctoral, Division of Chemistry & Chemical Engineering, Caltech

研究领域

Research in Professor Lin’s lab focuses on addressing fundamental technical problems related to molecular evolution of proteins, and on engineering proteins related to various bioprocesses. At present, there are three specific areas of research that are being pursued: (1) development of tools for directed evolution, for example, a high thorough-put autolysis scheme for E. coli cells; (2) evolution of useful biocatalysts and pathways, which includes engineering of enzymes and regulatory proteins related to biorefinery and for production of fine chemicals; (3) dissection and functional analysis of enzymes and viral proteins. Research in this lab has been supported by the national key basic research (973) program, the national high-tech (863) program, NSFC, and industrial grants. Prof. Lin also has been teaching an undergraduate course “principles and applications of genetic engineering.”

近期论文

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Lin, Z., Y. Zhang, J. Wang. Engineering of Transcriptional Regulators Enhances Microbial Stress Tolerance. Biotechnology Advances, 2013. DOI/10.1016/j.biotechadv.2013.02.010 (invited review). Ba, L., P. Li, H. Zhang, Y. Duan, Z. Lin. Semi-Rational Engineering of Cytochrome P450sca-2 in A Hybrid System for Enhanced Catalytic Activity: Insights into the Important Role of Electron. Biotechnology and Bioengineering, 2013. in press. Lin, Z., B. Zhou, W. Wu, L. Xing, Q. Zhao. Self-assembling amphipathic alpha-helical peptides induce formation of active protein aggregates in vivo. Faraday Discussions, 2013, in press (invited contribution) Wang, J., Y. Zhang, Y. Chen, M. Lin and Z. Lin. Global Regulator Engineering Significantly Improved Escherichia coli Tolerances toward Inhibitors of Lignocellulosic Hydrolysates. Biotechnology and Bioengineering, 2012. 109: 3133-3142. Chen, T., J. Wang, L. Zeng, R. Li, J. Li, Y. Chen and Z. Lin. Significant Rewiring of the Transcriptome and Proteome of an Escherichia coli Strain Harboring a Tailored Exogenous Global Regulator IrrE. PLoS ONE, 2012. 7: e37126. Xu, W.H., L. Han and Z. Lin. Screening of Random Peptide Library of Hemagglutinin from Pandemic 2009 A(H1N1) Influenza Virus Reveals Unexpected Antigenically Important Regions. PLoS ONE, 2011. 6: e18016. Xing, L., W. Wu, B.H. Zhou and Z. Lin, Streamlined Protein Expression and Purification Using Cleavable Self-Aggregating Tags. Microbial Cell Factories, 2011. 10: 42 ("highly accessed"). Wu, W., L. Xing, B.H. Zhou and Z. Lin. Active Protein Aggregates Induced by Terminally Attached Self-Assembling Peptide ELK16 in Escherichia coli. Microbial Cell Factories, 2011. 10: 9 ("highly accessed"). Lin, Z. and J. Zheng. Occurrence, Characteristics, and Applications of Fructosyl Amine Oxidases (Amadoriases). Applied Microbiology and Biotechnology, 2010. 86: 1613-1619 (invited review). Chen, Y., S. Li, T.J. Chen, H. Hua and Z. Lin. Random Dissection to Select for Protein Split Sites and Its Application in Protein Fragment Complementation. Protein Science, 2009. 18: 399-409. Chen, T.J., J.Q. Zhang, L. Liang, R. Yang and Z. Lin. An in vivo, Label-Free Quick Assay for Xylose Transport in Escherichia coli. Analytical Biochemistry, 2009. 390: 63-67. Cai, Z., W.H. Xu, R. Xue and Z. Lin. Facile, Reagentless and in situ Release of Escherichia coli Intracellular Enzymes by Heat-Inducible Autolytic Vector for High-Throughput Screening. Protein Engineering Design & Selection, 2008. 21: 681-687 (cover illustration) Joo, H., Z. Lin and F.H. Arnold. Laboratory Evolution of Peroxide-Mediated Cytochrome P450 Hydroxylation. Nature, 1999. 399: 670-673. Lin, Z. and E. Eisenstein. Nucleotide Binding-Promoted Conformational Changes Release a Nonnative Polypeptide from the Escherichia coli Chaperonin GroEL. Proceedings of the National Academy of Sciences of the United States of America, 1996. 93: 1977-1981.