个人简介
2009-present Professor, Nanjing University
2003-2009 Associated Professor, Nanjing University
2001-2002 Postdoctoral Associate in Max-Planck Institute of Colloids and Interfaces
1997-2001 PhD in Inorganic Chemistry, Coordination Chemistry Institute, Nanjing University
1994-1997 MSc. in Organic Chemistry, Nanjing University
1991-1994 R&D Chemical Engineer, Jiangyin Pesticide Company
1987-1991 BSc. in Chemistry, Nanjing University
南京大学化学化工学院教授、博士生导师。1991、1997和2001年分别获得南京大学化学专业学士、硕士和博士学位。2001-2002年在德国马普胶体与界面研究所从事博士后研究。2003年起在南京大学配位化学研究所工作。研究方向为无机信号小分子和生命关键过渡金属离子的探针设计、成像及其化学生物学；金属配合物的生物学效应，分子光物理、光化学效应的生物医药应用。在包括J. Am. Chem. Soc.; Angew. Chem. Int. Ed.; ACS Nano, Chem. Commun.; Chem. Sci.等国际知名学术刊物上发表论文近百篇。曾受邀为Chem. Soc. Rev 及Elsevier系列丛书撰写分子影像学综述和专章。先后主持国家自然科学基金面上项目6项，省面上项目1项；参加973项目2项，参加国家自然科学基金重点项目3项。曾获江苏省第五届优秀博士论文奖，2009年中美药协(SAPA)优秀研究生论文导师，2015年教育部自然科学奖一等奖（第2完成人）。
研究领域
Fluorescence imaging of signaling molecules Zn2+ and H2S
Zinc plays essential roles in signal transduction, neurotransmission, and gene transcription etc. Development of Zn2+ sensors to realize the subcellular Zn2+ imaging in specific organelles and the in vivo Zn2+ imaging in animal mode is our current interest, with the purpose to promote the understanding and theranostic application of Zn2+ biology. With the Zn2+ sensor developed in our lab, NBD-TPEA, the first in vivo Zn2+ imaging has been realized in live zebrafish larva, and the in vivo Zn2+ imaging in mammalian animal mode with the improved Zn2+ sensor is still undergoing. In addition, the subcellular Zn2+ imaging in mitochondria and nucleus with Zn2+ sensor of specific organelle-targetability has also been realized.
H2S was recognized as the third endogenous gasotransmitter after NO and CO. It can induce vasodilation, and displays the function of antioxidation, anti-apoptosis and anti-inflammation. The development of H2S probes of rapid response to realize the real time H2S tracking is especially required to acquire the real time information of endogenous H2S. A ratiometric H2S probe CouMC of quick response (<30 s) was prepared and the tracking of quick H2S enhancement in mitochondria (<80 s) upon NaHS incubation was realized with this probe.
Fluorescent tracking of paramagnetic metals in living cells
Overcoming the paramagnetic emission quenching effect to realize the in situ fluorescence imaging of Cu2+ and Fe3+ is challenging, and the electronic decoupling of metal center from fluorophore and reducing the metal paramagnetism with elaborately designed chelator are the reliable design rationales. Two ratiometric Cu2+ fluorescent sensors have been developed in our lab, and the ratiometric intracellular Cu2+ imaging has been realized. In addition, several Fe3+ sensors of turn-on response have been constructed with different sensing mechanism, and their reversible sensing ability over other reported Fe3+ sensors provides them the ability to track Fe3+ fluctuation in cells.
Tracking the exogenous toxic metal cations and metal-based drugs
Exogenous bioinorganic species are also closely associated with health. For example, Cd2+ and Hg2+ will lead to the irreversible damage to endocrine and nervous systems of human body. Overcoming the Zn2+ interference and the emission quenching effect of heavy atoms is the main task to construct fluorescent probes for Cd2+ and Hg2+ and realize the in vivo tracking and clarify their toxicology. The high sensitivity is another key point for these probes. High sensitive Hg2+ probe DT-ABD was designed based on the Hg2+-induced carbaldehyde recovery from 1,3-dithiane. With the detection limit of 4 nM (US EPA standard for drinking water 10 nM), the in vivo Hg2+ tracking in Hg2+-polluted zebrafish larva was realized using this probe. In addition, a ratiometric Cd2+ sensor DBITA of detection limit ~0.3 pM (US EPS standard for drinking water 40 nM) was developed, and its intracellular Cd2+ imaging ability was confirmed.
The cell and in vivo uptake, distribution and metabolism of metal-based anticancer agents are especially helpful to understand and improve their antitumor activity. Modifying these metal complexes with fluorophore has been confirmed to be a reliable means to acquire the above mentioned information via fluorescence imaging. With this method, the uptake, distribution of the chelating monofunctional Pt(II) complex in living cells and zebrafish larva have been investigated.
近期论文
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1. Q. Chen, H. Fang, X. Shao, Z. Tian, S. Geng, Y. Zhang, H. Fan, P. Xiang, J. Zhang, X. Tian, K. Zhang,* W. He,* Z. Guo,* J. Diao.* A dual-labeling probe to track functional mitochondria–lysosome interactions in live cells. Nat. Commun., 2020, 11, 6290.
2. M. A. Masood, Y. Chen*, S. Yao, S. Li, W. He,* Z. Guo.* A new palladium complex as a dual fluorometric and colorimetric probe for rapid determination of sulfide anion. J. Photochem. & Photobiol. A: Chem. 2021, 404,112885.
3. Y. Zhang, Y. Chen,* Y. Bai, X. Xue, W. He,* Z. Guo. FRET-based fluorescent ratiometric probes for the rapid detection of endogenous hydrogen sulphide in living cells. Analyst, 2020, 145, 4233–4238.
4. Y. Zhang, Y. Chen,* H. Fang, X. Shi, H. Yuan, Y. Bai, W. He,* Z. Guo. A ratiometric fluorescent probe for imaging enzyme dependent hydrogen sulfide variation in the mitochondria and in living mice. Analyst, 2020, 145, 5123–5127.
5. Z. Han, Y. Wang, Y. Chen,* H. Fang, H. Yuan, X. Shi, B. Yang, Z. Chen, W. He,* and Z. Guo.* A novel luminescent Ir(III) complex for dual mode imaging: synergistic response to hypoxia and acidity of the tumor microenvironment, Chem. Commun. 2020, 56, 8055-8058.
6. H. Xu, C. Zhu, Y. Chen,* Y. Bai, Z. Han, S. Yao, Y. Jiao, H. Yuan, W. He,* and Z. Guo.* A FRET-based fluorescent Zn2+ sensor: 3D ratiometric imaging, flow cytometric tracking and cisplatin-induced Zn2+ fluctuation monitoring. Chem. Sci., 2020,11, 11037–11041.
7. Y. Jiao, J. Hong, Y. Chen,* Y. Zhang, Z. Guo,* Z. Han and W. He.* A novel binuclear Pd(II) complex displaying synergic peptide cleavage behavior. Dalton Trans., 2020, 49, 3164–3173.
8. Z. Han, Y. Chen,* Y. Wang, X. Shi, H. Yuan, Y. Bai, Z. Chen, W. He,* Z. Guo.* Photoinduced synergistic cytotoxicity towards cancer cells via Ru(II) complexes. Dalton Trans., 2020, 49, 13954–13957.
9. J. Gao, Y. Chen,* Z. Guo, W. He.* Recent Endeavors on Molecular Imaging for Mapping Metals in Biology. Biophys. Rep.2020, 6(5),159–178.
10. J. Gao, Y. He, Y. Chen,* D. Song, Y. Zhang, Fen Qi, Z. Guo, W. He.* Reversible FRET Fluorescent Probe for Ratiometric Tracking of Endogenous Fe3+ in Ferroptosis. Inorg. Chem. 2020, 59, 10920−10927.
11. H. Fang, Y. Chen,* Y. Wang, S. Geng, S. Yao, D. Song1, W. He,* & Z. Guo.*A dual-modal probe for NIR fluorogenic and ratiometric photoacoustic imaging of Cys/Hcy in vivo. Sci. China, Chem., 2020, 63(5), 699–706.
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