Point-scanning microscopy approaches are transforming super-resolution imaging. Despite achieving parallel high-speed imaging using multifocal techniques, efficient multicolor imaging methods with high-quality illumination are currently lacking. In this paper, we present for the first time Mechanical-scan-free multiColor Super-resolution Microscopy (MCoSM) with spot array illumination, which enables mechanical-scan-free super-resolution imaging with adjustable resolution and a good effective field-of-view based on spatial light modulators. Through 100–2,500 s super-resolution spot illumination with different effective fields of view for imaging, we demonstrate the adjustable capacity of MCoSM. MCoSM extends existing spectral imaging capabilities through a time-sharing process involving different color illumination with phase-shift scanning while retaining the spatial flexibility of super-resolution imaging with diffractive spot array illumination. To demonstrate the prospects of MCoSM, we perform four-color imaging of fluorescent beads at high resolution. MCoSM provides a versatile platform for studying molecular interactions in complex samples at the nanoscale level.

点扫描显微镜方法正在改变超分辨率成像。尽管使用多焦点技术实现了并行高速成像，但目前缺乏具有高质量照明的高效多色成像方法。在本文中，我们首次提出了具有点阵列照明的无机械扫描多色超分辨率显微镜（MCoSM），它能够实现具有可调分辨率和良好有效视场的无机械扫描超分辨率成像。基于空间光调制器的视图。通过100-2,500秒不同有效视场的超分辨率光斑照明进行成像，我们展示了MCoSM的可调能力。 MCoSM 通过涉及不同颜色照明和相移扫描的分时过程扩展了现有的光谱成像功能，同时保留了衍射点阵列照明超分辨率成像的空间灵活性。为了展示 MCoSM 的前景，我们对荧光珠进行了高分辨率的四色成像。 MCoSM 提供了一个多功能平台，用于研究纳米级复杂样品中的分子相互作用。