β-Galactosidase (β-gal), an enzyme related to cell wall degradation, plays an important role in regulating cell wall metabolism and reconstruction. However, activatable fluorescence probes for the detection and imaging of β-gal fluctuations in plants have been less exploited. Herein, we report an activatable fluorescent probe based on intramolecular charge transfer (ICT), benzothiazole coumarin-bearing β-galactoside (), to achieve distinct in situ imaging of β-gal in plant cells. It exhibits high sensitivity and selectivity to β-gal with a fast response (8 min). can be used to efficiently detect the alternations of intracellular β-gal levels in cabbage root cells with considerable imaging integrity and imaging contrast. Significantly, can assess β-gal activity in cabbage roots under heavy metal stress (Cd, Cu, and Pb), revealing that β-gal activity is negatively correlated with the severity of heavy metal stress. Our work thus facilitates the study of β-gal biological mechanisms.

中文翻译：

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一种可激活荧光探针，用于重金属胁迫下卷心菜根部β-半乳糖苷酶活性的快速检测和原位成像

β-半乳糖苷酶（β-gal）是一种与细胞壁降解相关的酶，在调节细胞壁代谢和重建中发挥着重要作用。然而，用于植物中 β-gal 波动的检测和成像的可激活荧光探针的开发较少。在此，我们报告了一种基于分子内电荷转移（ICT）的可激活荧光探针，即带有苯并噻唑香豆素的β-半乳糖苷（），以实现植物细胞中β-半乳糖的独特原位成像。它对 β-gal 表现出高灵敏度和选择性，响应速度快（8 分钟）。可用于有效检测甘蓝根细胞内 β-gal 水平的变化，具有相当大的成像完整性和成像对比度。值得注意的是，可以评估重金属胁迫（Cd、Cu 和 Pb）下卷心菜根部的 β-gal 活性，揭示 β-gal 活性与重金属胁迫的严重程度呈负相关。因此，我们的工作促进了β-gal生物学机制的研究。