The prevention of lignin repolymerization/condensation in lignocellulose pretreatment can enhance both the bioconversion of cellulose and the quality of the obtained lignin. 2-Naphthol is a very effective pretreatment additive for suppressing lignin repolymerization, which enables overcoming the extraordinarily high recalcitrance of softwood to bioconversion. In this work, new approaches for adding 2-naphthol to the steam explosion pretreatment of softwood were studied. It was found that spray impregnation of spruce wood chips with 2-naphthol dissolved in acetone or ethanol is equally effective as the usually used soak-impregnation. However, spray-impregnation allows for an eight-fold reduction in solvent consumption. 2-Naphthol impregnation enabled practically quantitative cellulose conversion to glucose using an enzyme dosage of 30 FPU (filter paper units) g^–1 cellulose, which corresponds to a digestibility enhancement of 89.1% compared to the control without additive. It was further disclosed that spraying of water-suspended 2-naphthol onto wood chips enabled a high glucose yield of 81.9% using an enzyme dosage of 30 FPU g^–1 cellulose, which corresponds to an enhancement of 54.8% compared to the control and omits the use of solvents. An economic evaluation showed that spraying dissolved or water-suspended 2-naphthol is more cost-effective than soak-impregnation or admixing 2-naphthol flakes/powder to the biomass.

中文翻译：

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通过改进在蒸汽爆炸预处理中添加 2-萘酚来克服软木顽抗性

在木质纤维素预处理中防止木质素再聚合/缩合可以增强纤维素的生物转化和所得木质素的质量。 2-萘酚是一种非常有效的预处理添加剂，可抑制木质素再聚合，从而克服软木对生物转化的极高抵抗力。在这项工作中，研究了在针叶木蒸汽爆炸预处理中添加 2-萘酚的新方法。结果发现，用溶解在丙酮或乙醇中的 2-萘酚喷雾浸渍云杉木片与通常使用的浸泡浸渍同样有效。然而，喷雾浸渍可以将溶剂消耗减少八倍。使用 30 FPU（滤纸单位）g ^–1纤维素的酶剂量，2-萘酚浸渍几乎可以将纤维素定量转化为葡萄糖，与不含添加剂的对照相比，消化率提高了 89.1%。进一步披露，使用 30 FPU g ^–1纤维素的酶剂量，将水悬浮的 2-萘酚喷洒到木片上，可以实现 81.9% 的高葡萄糖产率，这相当于与对照相比提高了 54.8%，并且省略了溶剂的使用。经济评估表明，喷洒溶解或水悬浮的 2-萘酚比浸泡浸渍或将 2-萘酚薄片/粉末混合到生物质中更具成本效益。