STUDY QUESTION How do oocyte donors and recipients perceive the genetic link related to the transfer of nuclear DNA between donors and offspring? SUMMARY ANSWER Whether they are donors or recipients, individuals attach great importance to the transmission of their genetic heritage, since 94.5% would opt for the pronuclear transfer method to preserve this genetic link in the context of oocyte donation. WHAT IS KNOWN ALREADY Since 1983, the use of oocyte donation has increased worldwide. Performed in France since the late 1980s and initially offered to women with premature ovarian insufficiency, its indications have progressively expanded and now it is proposed in many indications to prevent the transmission of genetically inherited diseases. This has resulted in an increase in the waiting time for access to oocyte donation due to the difficulty in recruiting oocyte donors in French ART centres. Several articles have discussed how to fairly distribute donor oocytes to couples, but few have interviewed women in the general population to record their feelings about oocyte donation, as either the donor or recipient and the importance given to the genetic link between the oocyte donors and the children born. Mitochondrial replacement therapy (MRT) is a technique originally developed for women at risk of transmitting a mitochondrial DNA mutation. Recently, MRT has been considered for embryo arrest and oocyte rejuvenation as it could help females to reproduce with their own genetic material through the transfer of their oocyte nucleus into a healthy donor oocyte cytoplasm. STUDY DESIGN, SIZE, DURATION We conducted an opinion survey from January 2021 to December 2021, during which 1956 women completed the questionnaire. Thirteen participants were excluded from the analysis due to incomplete responses to all the questions. Consequently, 1943 women were included in the study. PARTICIPANTS/MATERIALS, SETTING, METHODS We specifically developed a questionnaire for this study, which was created and distributed using the Drag’n Survey® software. The questionnaire consisted of 21 items presented alongside a video created with whiteboard animation software. The aim was to analyse whether certain factors, such as age, education level, marital status, number of children, use of ART for pregnancy, video viewing, and knowledge about oocyte donation, were associated with feelings towards oocyte donation, by using a univariate conditional logistic regression model. This statistical method was also used to assess whether women would be more inclined to consider oocyte donation with the pronuclear transfer technique rather than the whole oocyte donation. All parameters found to be statistically significant in the univariate analysis were subsequently tested in a multivariate model using logistic regression. MAIN RESULTS AND THE ROLE OF CHANCE Most women were concerned about the biological genetic contribution of the donated oocyte (94.8%). The most common reason for a women's reluctance to donate their oocytes was their unwillingness to pass on their genetic material (33.3%). Nearly 70% of women who were initially hesitant to donate their oocytes indicated that they would reconsider their decision if the oocyte donation was conducted using donated cytoplasm and the pronuclear transfer technique. Concomitantly, >75% of the respondents mentioned that it would be easier to receive a cytoplasm donation. The largest proportion of the population surveyed (94.5%) expressed their support for its legalization. LIMITATIONS, REASONS FOR CAUTION In this study, a substantial portion of the responses came from individuals with medical or paramedical backgrounds, potentially introducing a recruitment bias among potential donors. The rate of missing responses to the question regarding the desire to become an oocyte donor was 13.6%, while the question about becoming an oocyte cytoplasm donor had a missing response rate of 23%. These missing responses may introduce a bias in the interpretation of the data. WIDER IMPLICATIONS OF THE FINDINGS This study was the first to demonstrate that, for the French population studied, the combination of oocyte cytoplasm donation with pronuclear transfer could offer a promising approach to enhance the acceptance of oocyte donation for both the donor and the recipient. STUDY FUNDING/COMPETING INTEREST(S) No external funding was used for this study. The authors have no conflicts of interest. TRIAL REGISTRATION NUMBER N/A.

中文翻译：

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捐赠者和接受者对卵母细胞捐赠的核 DNA 相关遗传遗产有何重视？

研究问题 卵母细胞捐赠者和接受者如何看待与捐赠者和后代之间核 DNA 转移相关的遗传联系？摘要答案 无论是捐赠者还是接受者，个人都非常重视其遗传遗产的传承，因为 94.5% 的人会选择原核移植方法来在卵母细胞捐赠的情况下保留这种遗传联系。已知信息 自 1983 年以来，卵母细胞捐赠的使用在世界范围内不断增加。自 20 世纪 80 年代末开始在法国进行，最初针对卵巢早衰的女性，其适应症逐渐扩大，现在在许多适应症中被提议用于预防遗传性疾病的传播。由于法国辅助生殖技术中心难以招募卵母细胞捐赠者，导致等待卵母细胞捐赠的时间增加。几篇文章讨论了如何将捐赠的卵母细胞公平地分配给夫妇，但很少有文章采访普通人群中的女性，记录她们作为捐赠者或接受者对卵母细胞捐赠的感受，以及对卵母细胞捐赠者和受赠者之间遗传联系的重视。孩子出生了。线粒体替代疗法 (MRT) 是一项最初为有传播线粒体 DNA 突变风险的女性开发的技术。最近，MRT 已被考虑用于胚胎停滞和卵母细胞返老还童，因为它可以通过将卵母细胞核转移到健康供体卵母细胞的细胞质中，帮助女性用自己的遗传物质进行繁殖。研究设计、规模、持续时间 我们于 2021 年 1 月至 2021 年 12 月进行了一项民意调查，期间有 1956 名女性完成了调查问卷。由于对所有问题的回答不完整，十三名参与者被排除在分析之外。因此，1943 名女性被纳入该研究。参与者/材料、设置、方法 我们专门为本研究开发了一份调查问卷，该调查问卷是使用 Drag'n Survey® 软件创建和分发的。该调查问卷包含 21 个项目，并附有使用白板动画软件创建的视频。目的是通过使用单变量分析某些因素，例如年龄、教育水平、婚姻状况、子女数量、妊娠使用 ART、视频观看和卵母细胞捐赠知识等是否与卵母细胞捐赠的感受相关。条件逻辑回归模型。这种统计方法还用于评估女性是否更倾向于考虑采用原核移植技术捐献卵母细胞而不是全卵母细胞捐献。随后使用逻辑回归在多变量模型中测试在单变量分析中具有统计显着性的所有参数。主要结果和机会的作用 大多数女性担心捐赠卵母细胞的生物遗传贡献（94.8%）。女性不愿捐献卵母细胞的最常见原因是她们不愿意传递遗传物质（33.3%）。近 70% 最初对捐献卵母细胞犹豫不决的女性表示，如果使用捐献的细胞质和原核移植技术进行卵母细胞捐献，她们会重新考虑自己的决定。与此同时，超过 75% 的受访者提到接受细胞质捐赠会更容易。最大比例的受访人口（94.5%）表示支持其合法化。局限性和谨慎的原因在这项研究中，很大一部分回复来自具有医学或辅助医学背景的个人，这可能会在潜在捐赠者中引入招募偏见。关于是否愿意成为卵母细胞捐献者的问题的漏答率为13.6%，而对于成为卵母细胞细胞质捐献者的问题的漏答率为23%。这些缺失的响应可能会在数据解释中引入偏差。研究结果的更广泛意义这项研究首次证明，对于所研究的法国人群来说，卵母细胞胞质捐赠与原核移植的结合可以提供一种有前景的方法，以提高捐赠者和接受者对卵母细胞捐赠的接受度。研究资助/竞争利益 本研究未使用外部资助。作者没有利益冲突。试用注册号 不适用。作者没有利益冲突。试用注册号 不适用。作者没有利益冲突。试用注册号 不适用。