Background Non-severe paediatric burns can result in poor long-term health outcomes. This occurs even in cases with good acute burn-related outcomes, including minimal scarring. The mechanisms that underpin the transition from non-severe burn to sustained negative long-term health impacts are currently unknown. However, sustained metabolic and immune changes have been observed in paediatric burn studies, suggesting these changes may be important. The plasma lipidome consists of a rich pool of bioactive metabolites that play critical roles in systemic processes including molecular signalling and inflammation. We hypothesised that changes in the plasma lipidome may reflect underlying changes in health status and be linked to long-term health after burn trauma. Methods This study analysed the lipidome in children who had previously experienced a non-severe burn, compared to non-injured controls. Thirty-three participants were recruited between the ages of 5 and 8 years who had experienced a non-severe burn between the ages of 1 and 3 years. Plasma samples were also collected from a non-injured, healthy, age and gender matched control group (n = 21). Plasma lipids were measured using reversed-phase liquid chromatographymass spectrometery (LC-MS). Results In total 838 reproducible lipid species from 19 sub-classes passed quality control procedures and progressed to statistical analysis. Analysis of individual lipid metabolites showed significantly higher concentrations of lysophosphatidylethanolamines and phosphatidylethanolamines, and significantly lower concentrations in myristic, palmitic and palmitoleic acids in the plasma of those who had experienced burn injury compared to controls. Conclusion Long-term changes in the lipid profile may give insight into the mechanisms underlying poor long-term health subsequent to non-severe burn injury. Further work to investigate the relationship between long-term pathology and lipidomic changes may lead to a better understanding of the causes of secondary morbidity post-burn and to clinical intervention to reduce the long-term health burden of burn trauma.

中文翻译：

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血浆脂质组学揭示儿童烧伤后整个早期生命中持续存在的系统变化

背景 非严重儿童烧伤可能导致长期健康结果不佳。即使在急性烧伤相关结果良好（包括疤痕最小）的情况下，也会发生这种情况。目前尚不清楚从非严重烧伤到持续的长期负面健康影响转变的机制。然而，在儿科烧伤研究中观察到持续的代谢和免疫变化，表明这些变化可能很重要。血浆脂质组由丰富的生物活性代谢物组成，在分子信号传导和炎症等全身过程中发挥着关键作用。我们假设血浆脂质组的变化可能反映了健康状况的潜在变化，并与烧伤后的长期健康有关。方法本研究分析了先前经历过非严重烧伤的儿童与未受伤对照儿童的脂质组。招募了 33 名年龄在 5 岁到 8 岁之间的参与者，他们在 1 岁到 3 岁之间经历过非严重烧伤。还从未受伤、健康、年龄和性别匹配的对照组（n = 21）中收集血浆样本。使用反相液相色谱质谱法（LC-MS）测量血浆脂质。结果 来自 19 个亚类的总共 838 种可重复脂质种类通过了质量控制程序并进行了统计分析。对个体脂质代谢物的分析显示，与对照组相比，经历过烧伤的患者血浆中溶血磷脂酰乙醇胺和磷脂酰乙醇胺的浓度显着较高，而肉豆蔻酸、棕榈酸和棕榈油酸的浓度显着较低。结论 血脂谱的长期变化可能有助于了解非严重烧伤后长期健康状况不佳的机制。进一步研究长期病理学与脂质组学变化之间的关系可能有助于更好地了解烧伤后继发发病的原因，并进行临床干预以减轻烧伤创伤的长期健康负担。