Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores,Analytical Chemistry

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Analysis of Pore Formation and Protein Translocation Using Large Biological Nanopores
Analytical Chemistry ( IF 6.7 ) Pub Date : 2017-10-23 00:00:00 , DOI: 10.1021/acs.analchem.7b01550
Hirokazu Watanabe ₁ , Alberto Gubbiotti ₂ , Mauro Chinappi ₃ , Natsumi Takai ₁ , Koji Tanaka ₄ , Kouhei Tsumoto ₄ , Ryuji Kawano ₁

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This paper describes the analysis of pore formation and detection of a single protein molecule using a large nanopore among five different pore-forming proteins. We demonstrate that the identification of appropriate pores for nanopore sensing can be achieved by classifying the channel current signals and performing noise analysis. Through these analyses, we selected a perforin nanopore from the membrane attack complex/perforin superfamily and attempted to use it to detect the granzyme B protein, a serine protease. As a result, we found that granzyme B might pass through the perforin nanopore if it adopts an unfolded structure. Our proposed analytical approach should be useful for exploring several types of nanopore as large biological nanopores other than α-hemolysin.

中文翻译：

使用大的生物纳米孔分析毛孔形成和蛋白易位。

本文介绍了孔的形成分析和使用五个不同的成孔蛋白之间的大纳米孔检测单个蛋白分子的方法。我们证明，通过对通道电流信号进行分类并进行噪声分析，可以实现对纳米孔传感的合适孔的识别。通过这些分析，我们从膜攻击复合物/穿孔素超家族中选择了穿孔素纳米孔，并试图将其用于检测粒酶B蛋白（一种丝氨酸蛋白酶）。结果，我们发现颗粒酶B如果采用未折叠的结构，则可能会穿过穿孔素纳米孔。我们提出的分析方法对于探索几种类型的纳米孔应该有用，如α-溶血素以外的大型生物纳米孔。

更新日期：2017-10-23

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