Rheumatoid arthritis (RA) is an autoimmune disease with an unknown etiology, occurring in approximately 1.0% of general population. More and more studies have suggested that long non-coding RNAs (lncRNAs) could play important roles in various biological processes and be associated with the pathogenesis of different kinds of diseases including RA. Although a large number of lncRNAs have been found, our knowledge of their function and physiological/pathological significance is still in its infancy. In order to reveal functional lncRNAs and identify the key lncRNAs in RA, we reconstructed a global triple network based on the competitive endogenous RNA (ceRNA) theory using the data from National Center for Biotechnology Information Gene Expression Omnibus and our previous paper. Meanwhile, Gene Ontology (GO) and pathway analysis were performed using Cytoscape plug-in BinGO and Database for Annotation, Visualization, and Integration Discovery (DAVID), respectively. We found that the lncRNA–miRNA–mRNA network was composed of 7 lncRNA nodes, 90 mRNA nodes, 24 miRNA nodes, and 301 edges. The functional assay showed that 147 GO terms and 23 pathways were enriched. In addition, three lncRNAs (S5645.1, XR_006437.1, J01878) were highly related to RA, and therefore, were selected as key lncRNAs. This study suggests that specific lncRNAs are associated with the development of RA, and three lncRNAs (S5645.1, XR_006437.1, J01878) could be used as potential diagnostic biomarkers and therapeutic targets.

中文翻译：

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基于竞争性内源RNA的lncRNA–miRNA–mRNA网络的重建和分析揭示了类风湿关节炎中的功能性nccRNA

类风湿关节炎（RA）是一种病因不明的自身免疫性疾病，约占总人口的1.0％。越来越多的研究表明，长的非编码RNA（lncRNA）可能在各种生物学过程中发挥重要作用，并与包括RA在内的多种疾病的发病机制有关。尽管已经发现了大量的lncRNA，但我们对其功能和生理/病理学意义的了解仍处于起步阶段。为了揭示RA中功能性lncRNA并鉴定关键的lncRNA，我们使用竞争性内源RNA（ceRNA）理论，使用国家生物技术信息基因表达综合中心和我们之前论文的数据，构建了一个全球三重网络。同时，分别使用Cytoscape插件BinGO和用于注释，可视化和集成发现的数据库（DAVID）进行了基因本体论（GO）和途径分析。我们发现lncRNA–miRNA–mRNA网络由7个lncRNA节点，90个mRNA节点，24个miRNA节点和301个边缘组成。功能测定显示147个GO术语和23个途径被富集。另外，三个lncRNA（S5645.1，XR_006437.1，J01878）与RA高度相关，因此被选为关键lncRNA。这项研究表明特定的lncRNA与RA的发展有关，并且三种lncRNA（S5645.1，XR_006437.1，J01878）可以用作潜在的诊断生物标志物和治疗靶标。我们发现lncRNA–miRNA–mRNA网络由7个lncRNA节点，90个mRNA节点，24个miRNA节点和301个边缘组成。功能测定显示147个GO术语和23个途径被富集。另外，三个lncRNA（S5645.1，XR_006437.1，J01878）与RA高度相关，因此被选为关键lncRNA。这项研究表明特定的lncRNA与RA的发展有关，并且三种lncRNA（S5645.1，XR_006437.1，J01878）可以用作潜在的诊断生物标志物和治疗靶标。我们发现lncRNA–miRNA–mRNA网络由7个lncRNA节点，90个mRNA节点，24个miRNA节点和301个边缘组成。功能测定显示147个GO术语和23个途径被富集。另外，三个lncRNA（S5645.1，XR_006437.1，J01878）与RA高度相关，因此被选为关键lncRNA。这项研究表明特定的lncRNA与RA的发展有关，并且三种lncRNA（S5645.1，XR_006437.1，J01878）可以用作潜在的诊断生物标志物和治疗靶标。